Pregnant women can be subject to various types of infection that can have an impact on both the pregnancy and foetal health. Various guidelines recommend carrying out serological tests during pregnancy in order to identify and treat them. The data on serological screening during pregnancy are recorded on the birth attendance certificate (CEDAP) by the midwives, using an electronic template, at all maternity facilities in the province of Trento (north east Italy). The annual birth attendance certificate databases for all pregnant women who received care from 01.01.2015 and 31.12.2019 have been analysed. For cases in which a positive result, results pending or seroconversion were recorded, the hospital information system was consulted to acquire information on the result of the test, and any treatments prescribed. Serological screening coverage and seroprevalence were calculated for Rubella, Cytomegalovirus (CMV), HBV, HCV, HIV, Toxoplasmosis, Syphilis, group B Streptococcus (GBS) infection. A total of 20,660 pregnant women were analysed. Average cover for all of the serological tests during the period considered was 97.5%, and no differences were observed with regard to the age group, academic qualifications and nationality of the pregnant women, or with regard to the maternity facility. The average seroprevalence of CMV virus was 69.5/100 pregnant women tested,; for HBV it was 0.76/100, for HCV it was 0.39/100, for HIV it was 0.15/100, for Rubella virus it was 90.5/100, for Toxoplasmosis it was, 21.7/100, for Syphilis it was 0.2/100, and for GBS it was 21/100. The seroprevalence values followed an upward trend with a decrease in the pregnant woman’s academic qualifications and were higher among foreign nationals. The coverage for serological screening shows extensive compliance with the indications provided by Italian guidelines and homogeneous management throughout the province of Trento. Foreign women and women with a low level of schooling warrant special attention.