Introduction: Generation of Biomedical Waste (BMW) in healthcare facilities is an unavoidable factor for the clinical practices and care of patients. The frequent use of disposable materials in the hospital enhances the amount of generation of BMW. It is necessary to manage BMW compliance with Biomedical Waste Management (BMWM) Rules 2016 to avoid any type of public health hazards and environmental pollution.

Objectives: The study intends to find out the knowledge and attitude of the HCWs towards the IC protocols compliance related to segregation of BMW in Katihar Medical College (KMC).

Material and method: This study consists of observation by using observational checklist including various parameters in six different departments in KMC for BMWM for 40 days. The questionnaire having 10 MCQs was used to know about the socio-demographic characteristics, knowledge and attitude of 121 participants towards the segregation of BMW according to BMWM Rules,2016.

Results: The infection control protocols were least followed for white bins regarding all the observational parameters except bins with closed fitting cover and the availability of blue bins with closed fitting cover was least i.e. only 86 (35.83%) in total 240 observations. The awareness level of the participants towards the segregation of BMW in the OBG Department is very high, whereas the overall awareness level is low.

Conclusion: The study reveals that the availability of colour coded bins in the departments where research was done is not followed properly. The overall knowledge and attitude about segregation of BMW among the participants was low. The study suggests the recommendation of conducting periodic in-service training among the existing staff and for the new staff at the time of induction. The proper training record should be maintained so that all the staff would train effectively and efficiently time to time.

Priya has completed her graduation in B.Tech. in electronics and communication engineering from Millia Institute of Technology, Rambagh, Purnia, Bihar, India. After that, she worked as an insurance advisor at National Insurance Co. Ltd. for three years and as a senior computer operator at Katihar Medical College for one year. She then completed her master’s degree in Healthcare Administration (MHA) from Al-Karim University, Katihar–854105, Bihar, India, in 2025.